Laboratorio de Farmacología

Somos un equipo de investigadores que hemos unido nuestras capacidades para realizar investigación básica y aplicada, en el ámbito de la farmacología, incluida la realización de ensayos de bioactividad de sustancias provenientes de diversas fuentes (naturales, sintéticas y/o semisintéticas), incluido los alimentos. Nuestro propósito es aportar nuevas herramientas para el diagnóstico, prevención y/o tratamiento de enfermedades. Nuestras colaboraciones se extienden a través de universidades y centros de investigación, nacionales y extranjeros. Destacamos las colaboraciones con investigadores de la Pontificia Universidad Católica de Valparaíso, del Instituto Max-Plank (MPI), Dormund, Alemania, y del Instituto Teófilo Hernando de I+D del Medicamento, Universidad Autónoma de Madrid, España.

Servicios

Ensayos de Bioactividad

El Laboratorio de Farmacología, como parte del Centro Regional de Estudios en Alimentos Saludables (CREAS), dispone de una amplia gama de ensayos in vitro e in vivo que tienen como propósito evaluar el potencial bioactivo de compuestos sintéticos, semisintéticos, componentes de alimentos, y en general productos naturales de diversos orígenes.

blue-pills-in-petri-dish-pharmacology-research-concept

Entre los ensayos de bioactividad disponibles se encuentran: actividad estimulante y depresora del sistema nervioso central, analgésica, antiinflamatoria, vasoconstrictora, vasodilatadora, antiagregante plaquetaria, hipoglicemiante, hipolipidémica, antiproliferativa y pre/probiótica.

Lab_Fgia_01 mk_01Algunos ensayos -como aquellos que involucran fitoquímicos- son realizados en diferentes niveles de complejidad biológica, desde el nivel molecular (ej. expresión génica) al nivel funcional, pasando por ensayos en cultivos celulares, órganos aislados, modelos animales y ensayos clínicos.

Se cuenta con un robusto set de ensayos que nos permite determinar un “índice antioxidante” y un “índice protector”, orientados a evaluar de forma integral actividad antioxidante y protectora, respectivamente.mk_ag_lab_01

Este set de ensayos contempla determinaciones de bioactividad desde el nivel molecular al organismo vivo, e incluye capacidad antioxidante total, protección vascular (vasoprotección), protección neuronal (neuroprotección) y protección inducida por fitoquímicos en modelo animal de estrés oxidativo.

EQUIPAMIENTO COLABORATIVO

Equipamiento FONDEQUIP 2016

Fortalecimiento de la Investigación Colaborativa en la Región de Valparaíso para la Identificación de Moléculas Bioactivas de Interés Medicinal o Biotecnológico Mediante Técnicas de Cribado de Alto Rendimiento y Alto Contenido.

Equipamiento UV – Centro Regional de Estudios en Alimentos y Salud (CREAS)

  • Lector de placas multidetector
  • Equipamiento de biología molecular
  • Baño de órganos para ensayos in vitro

Equipamiento UV – Max Planck Institute (Dormund, Alemania)

  • Equipo de patch-clamp completo y operativo montado sobre microscopio Zeiss Axiovert A1
  • Cámara de Bioseguridad

Equipamiento Fondecyt – DIPUV

  • Equipo de microespectrofluorimetría montado sobre microscopio Nikon y acoplado a cámara digital enfriada para obtención de imágenes en tiempo real.
  • Microscopio Nikon invertido
  • Incubador CO2
  • Múltiples equipos menores

PUBLICACIONES

ARTÍCULOS INDEXADOS EN WEB OF SCIENCE
• Valdés C, Laurido C, Morales B, Jaimes L, Vinet R, Martínez JL. 2020. Complete essential oils of Laurus nobilis inducing antinociceptive action by opioid mechanism in C-Reflex and spinal Wind-Up model in rat. B Latinoam Caribe Pl 19:420-7. doi: 10.37360/blacpma.20.19.4.29
• Martínez JL, Bruzzone ME, Morales B, Palacios J, Jaimes L, Knox M, Vinet R, Laurido C. 2019. Effects of Indomethacin on the Norepinephrine-Induced Contraction in Vascular Smooth Muscle in Different Stages of the Oestrous Cycle. Pak Vet J. 39(3): 443-446. doi: 10.29261/pakvetj/2019.093.
• Knox M, Vinet R, Fuentes L, Morales B, Martínez JL. 2019. A Review of Endothelium-Dependent and -Independent Vasodilation Induced by Phytochemicals in Isolated Rat Aorta. Animals (Basel) 2019, 9, 623; doi:10.3390/ani9090623.
• Fuentes L, Figueroa CR, Valdenegro M, Vinet R. 2019. Patagonian berries: healthy potential and the path to becoming functional foods. Foods 2019, 8, 289; doi: 10.3390/foods8080289
• Molinari A, Oliva A, Marlene Arismendi-Macuer M, Guzmán L, Acevedo W, Aguayo D, Vinet R, San Feliciano A. 2019. Antiproliferative Benzoindazolequinones as Potential Cyclooxygenase-2 inhibitors. Molecules 24(12), 2261. doi.org/10.3390/molecules24122261
• Jaimes L, Vinet R, Knox M, Morales B, Benites J, Laurido C, Martínez JL. 2019. A Review of the Actions of Endogenous and Exogenous Vasoactive Substances during the Estrous Cycle and Pregnancy in Rats, Animals (Basel). 2019 May 29;9(6). pii: E288. doi: 10.3390/ani9060288.
• Martinez JL, Benites J, Gomez H, Vargas-Rueda S, Vinet R, Jaimes L, Laurido, C. 2018. Effects of two bisbenzylisoquinoline alkaloids, Antioquine and Tetrandrine, compared to Verapamil in Rat Thoracic Aorta. An Acad Bras Cienc 90:1459-63
• Guzman L, Balada C, Flores G, Alvarez R, Knox M, Vinet R, Martínez JL. 2018. t-Resveratrol Protects against Acute High Glucose Damage in Endothelial Cells. Plant Food Hum Nutr 73:235-40. doi: 10.1007/s11130-018-0683-0.
• Martinez JL, Rovirosa J, Laurido C, San Martin A, Vinet R, Jaimes L. 2017. The Pulmonate Limpet Siphonaria lessoni: Pharmacological Study of Norsiphonarienone, a Polipropionate. Pak Vet J 37:482-4
• Vinet R, Alvarez R, Knox M, Guzman L, Martinez JL, Flores E. 2016. Vasodilatory properties of Solanum crispum Ruiz & Pav. a South American native plant. B Latinoam Caribe Pl 15:94-8
• Mahmood MS, Martinez JL, Aslam A, Rafique A, Vinet R, Laurido C, Hussain I, Abbas RZ, Khan A, Ali S. 2016. Antiviral Effects of Green Tea (Camellia Sinensis) against Pathogenic Viruses in Human and Animals (a Mini-Review). Afr J Tradit Complem 13:176-84
• Fuentes L, Valdenegro M, Gomez MG, Ayala-Raso A, Quiroga E, Martínez JP, Vinet R, Figueroa C. 2016. Characterization of fruit development and potential health benefits of arrayan (Luma apiculata), a native berry of South America. Food Chem 196:1239-47
• Prieto J, Cortes D, Jaimes L, Laurido C, Vinet R, Martinez JL. 2015. Pseudoxandra sclerocarpa Maas, Colombian medicinal plant: a review. B Latinoam Caribe Pl 14:308-16
• Molinari A, Oliva A, Arismendi-Macuer M, Guzman L, Fuentealba M, Knox M, Vinet R, San Feliciano A. 2015. New 1H-Benzo[f]indazole-4,9-diones Conjugated with C-Protected Amino Acids and Other Derivatives: Synthesis and in Vitro Antiproliferative Evaluation. Molecules 20:21924-38
• Vinet R, Cortes MP, Alvarez R, Delpiano MA. 2014. Bradykinin and histamine-induced cytosolic calcium increase in capillary endothelial cells of bovine adrenal medulla. Cell Biol Int 38:1023-31
• Vinet R, Araos P, Gentina JC, Knox M, Guzman L. 2014. p-Coumaric acid reduces high glucose-mediated impairment of endothelium-dependent relaxation in rat aorta. B Latinoam Caribe Pl 13:232-7
• Sotomayor-Zarate R, Jara P, Araos P, Vinet R, Quiroz G, Renard GM, Espinosa P, Hurtado-Guzmán C, Moya PR, Iturriaga-Vásquez P, Gysling K, Reyes-Parada M. 2014. Improving Amphetamine Therapeutic Selectivity: N,N-dimethyl-MTA has Dopaminergic Effects and does not Produce Aortic Contraction. Basic Clin Pharmacol 114:395-9
• Barraza F, Calvo C, Silva M, Vinet R, Laurido C, Barrera E, Meza I, Vargas-Rueda S, Martínez JL. 2014. Traditional ethnobotanical knowledge and use of medicinal plants in a rural area of the metropolitan region of Chile: descriptive study. B Latinoam Caribe Pl 13:366-74
• Alvarez R, Knox M, Guzman L, Vinet R. 2014. T-Resveratrol Protects Endothelium-Dependent Relaxation in Isolated Rat Aorta Acutely Exposed to High Glucose. Basic Clin Pharmacol 115:46
• Cortes MP, Becerra JP, Vinet R, Alvarez R, Quintana I. 2013. Inhibition of ATP-induced calcium influx by homocysteine in human umbilical vein endothelial cells. Cell Biol Int 37:600-7
• Vinet R, Knox M, Mascher D, Paredes-Carbajal C, Martinez JL. 2012. Isolated aorta model and its contribution to phytopharmacology. B Latinoam Caribe Pl 11:35-45
• Vinet R, Cortes M, Alvarez R, Guzman L, Flores E. 2012. Centaurium cachanlahuen (Mol.) Robinson, a Chilean native plant with a vasodilatory effect. B Latinoam Caribe Pl 11:61-5
• Guzman L, Depix MS, Salinas AM, Roldan R, Aguayo F, Silva A, Vinet R. 2012. Analysis of aberrant methylation on promoter sequences of tumor suppressor genes and total DNA in sputum samples: a promising tool for early detection of COPD and lung cancer in smokers. Diagn Pathol 7:87
• Vinet R, Cortes M, Luxoro M, Delpiano MA. 2009. Nicotine-evoked cytosolic Ca2+ increase and cell depolarization in capillary endothelial cells of the bovine adrenal medulla. Biol Res 42:111-9
• Vinet R, Gonzalez J, Hidalgo V, Luxoro M, Delpiano MA, Cortes M. 2006. Bradykinin and histamine-induced cytosolic Ca2+ rise in bovine adrenal microvascular endothelial cells. J Vasc Res 43:552
• Luxoro M, Vinet R. 2001. Physiological bases for an interaction between chromaffin and endothelial cells from the adrenal gland. Rev Chil Hist Nat 74:39-45
• Vinet R, Rojas F, Luxoro M, Vargas F, Cortes M. 2000. Catecholamines-evoked cytosolic Ca2+ rise in endothelial cells from bovine adrenal medulla. Mol Cell Biochem 203:53-8
• Vinet R, Flores N, Bustos P, Lutz M. 2000. Effect of dietary oils on endothelium-dependent and -independent relaxation in rat aorta. J Physiol-London 523:106
• Delpiano MA, Vinet R, Cortes M. 2000. Ca2+ channels and cytosolic Ca2+ in endothelial cells of brain and adrenal medulla microvessels. Eur J Neurosci 12:387
• Cortes M, Rodriguez S, Vinet R, Luxoro M. 2000. Nicotine-evoked changes in cytosolic calcium and membrane currents in cultured bovine adrenal medulla endothelial cells. J Physiol-London 523:34
• Vinet R, Vargas FF. 1999. L- and T-type voltage-gated Ca2+ currents in adrenal medulla endothelial cells. Am J Physiol-Heart C 276:H1313-H22
• Vinet R, Rojas F, Cortes M, Luxoro M, Vargas F. 1998. Catecholamines-evoked cytosolic Ca2+ ([Ca2+]) rise in bovine adrenal medulla endothelial cells (BAMECs). Faseb J 12:A104
• Goping G, Kuijpers GAJ, Vinet R, Pollard HB. 1996. Comparison of LR White and Unicryl as embedding media for light and electron immunomicroscopy of chromaffin cells. J Histochem Cytochem 44:289-95
• Lutz M, Cortez J, Vinet R. 1995. Effects of dietary fats, alpha-tocopherol and beta-carotene supplementation on aortic ring segment responses in the rat. Int J Vitam Nutr Res 65:225-30
• Vargas FF, Vinet R, Calvo S. 1994. Voltage-Gated Ca++ Channels in Adrenal-Medulla Endothelial-Cells and Their Loss during Cell-Culture. Faseb J 8:A1061
• Montiel C, Delafuente MT, Vinet R, Delvalle M, Gandia L, et al. 1994. Interactions between Ca2+, Pca50941 and Bay-K-8644 in Bovine Chromaffin Cells. Eur J Pharm-Molec Ph 268:293-303
• Lutz M, Cortez J, Vinet R. 1994. Dietary Fats and Cholesterol Supplementation Effects on Aortic and Lipid Response in Rats. J Nutr Biochem 5:446-50
• Pinardi G, Brieva C, Vinet R, Penna M. 1992. Effects of Chronic Ethanol Consumption on Alpha-Adrenergic-Induced Contractions in Rat Thoracic Aorta. Gen Pharmacol 23:245-8
• Vinet R, Brieva C, Pinardi G, Penna M. 1991. Modulation of Alpha-Adrenergic-Induced Contractions by Endothelium-Derived Relaxing Factor in Rat Aorta. Gen Pharmacol 22:137-42
• Vinet R, Brieva C, Pinardi G, Penna M. 1991. Influence of Extracellular Ca2+ on the Modulation of Alpha-Adrenergic-Induced Contractions in Rat Aorta. Gen Pharmacol 22:365-9
• Vinet R, Pinardi G. 1988. Endothelial Role in Alpha-Adrenergic Induced Vasoconstriction in Rat Thoracic Aorta. Acta Cient Venez 39:340-3
• Vinet R, Palomino M, Acuna P, Pinardi J. 1987. Effect of Endothelial Removal in the Contractile Response to Alfa-Adrenergic Agonist in Rat Aorta. Arch Biol Med Exp 20:R257
• Fernandez E, Pena W, Vinet R, Hidalgo ME. 1986. Kinetics of Photodegradation of Nalidixic-Acid. An Quim a-Fis Tec 82:96-8

CAPÍTULO DE LIBROS
• Martínez A, Jaimes L, Vinet R, Segura T, Laurido C, Martínez JL. 2019. Native Medicinal Plants Used for the Treatment of Nervous System Ailments in Chile and the Current State of Its Scientific Studies. In: Martínez, Muñoz-Acevedo & Rai. Ethnobotany: Local Knowledge and Traditions. CRC Press. doi: 10.4103/0253-7613.108331
• Vinet R, Knox M, Lorca M, Laurido C, Matínez JL. 2018. In Vitro Experimental Design and Data Analysis in Ethnopharmacology. In: Ray & Martínez. Ethnobotany: Application of Medicinal Plants. CRC Press.
• Vinet R, Martínez JL, Guzmán L. 2015. The Therapeutic Potential of Products Based on Polyphenols from Wine Grapes. In: Duarte & Rai. Therapeutic Medicinal Plants: from Lab to the Market. Duarte & Rai editors. CRC Press.
• Escalona JC, Peres-Roses R, Rodriguez, JR, Laurido C, Vinet R, Lafourcade A, Jaimes L, Martinez JL. 2015. The Cuban Traditional Experience in Developing Products Based on Medicinal Plants. In: Duarte & Rai.Therapeutic Medicinal Plants: from Lab to the Market. CRC Press.
• Cortés MP, Alvarez R, Maldonado J, Vinet R, Barría K. 2013. Monoclonal Gammopathy of Undetermined Significance, Multiple Myeloma – A Quick Reflection on the Fast Progress, Prof. Roman Hajek (Ed.), ISBN: 978-953-51-1083-5, InTech, DOI: 10.5772/56138.
• Vargas F, Calvo S, Vinet R, Rojas E. 1998. Interactions between bovine adrenal medulla endothelial cells and chromaffin cell. In: Bassingthwaighte & Linehan. Whole organ approach to cellular metabolism. Springer, NY, USA. pp 91-107.
• Lutz M, Topasio A, Acuña P, Vinet R. 1993. Comparative effects of sardine and olive oils intake and cholesterol supplementation on serum, liver lipids and vascular smooth muscle reactivity in rats. In: Drevon, Baksaas & Krokan. Omega-3 Fatty Acids. Birkhauser, Verlag, pp.183-190.

OTROS ARTÍCULOS
• Martinez JL, Russo A, Vinet R, Jaimes L, Russo L, Laurido C. 2018. Berberis chilensis gillies ex hook: Alkaloids and pharmacological activities. PharmacologyOnLine 1: 58-67.
• Martinez JL, Vinet R, Laurido C, Jaimes L, Vargas-Rueda S, Rovirosa J, San-Martin, A. 2016. Effect on rat blood pressure of epitaondiol, diterpenoid isolated from the algae Stypopodium flabeliforme of Rapa Nui (Chilean island territory in the pacific ocean). PharmacologyOnLine 3: 209-212.
• Vinet R, Alvarez R, Knox M, Guzman L. 2015. Bioactivity and therapeutic potential of resveratrol and derivatives on the cardiovascular system. Rev Farmacol Chile 8: 52-61.
• Vinet R, Knox M, Marinovic M. 2013. Tecnologías de información y comunicación, complejidad y educación: nuevos desafíos y oportunidades para el aprendizaje de la farmacología. Rev Farmacol Chile 6: 19-26.


DR. RAÚL VINET HUERTA

  • PhD . Ciencias Biomédicas
  • MSc – Farmacología
  • MBA – RRHH – Marketing
  • Pos Doc – National Institutes of Health (NIH, NIDDK, USA)